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Hence, the present investigation was carried out to estimate the phytochemical properties and antioxidant activity of 80% ethanol of Rhodomyrtus tomentosa leaves

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In Vitro Antioxidant Activity of Rhodomyrtus tomentosa leave by 80% Ethanol Extraction

By

NORLELA BT SAMSUDIN

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ii

DECLARATION

I hereby declare that this thesis is my original work and has not been submitted previously or currently for any other degree at UiTM or any other institutions

Signature,

Name: Norlela Binti Samsudin Matric Number: 2014627124 Date: July 11, 2017

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ABSTRACT

In vitro antioxidant activity of Rhodomyrtus tomentosa leave by 80% ethanol extraction

A wide range of phytochemicals present in leaves of Rhodomyrtus tomentosa act as antioxidant agents and play an important role in protection against free radical. Hence, the present investigation was carried out to estimate the phytochemical properties and antioxidant activity of 80% ethanol of Rhodomyrtus tomentosa leaves. Phytochemical properties of Total Phenolic Compound (TPC) were determined by Follin-Ciocalteu method and for Flavonoid Compound (TFC) by Aluminium Chloride Cholorimetric Assay. While antioxidant activities was determined by Total Ferric Reducing Antioxidant Power (FRAP) and 2, 2-diphenyl- 1-picryl-hydrazyl-hydrate (DPPH) scavenging activity. In 1 mg/ml of Rhodomyrtus tomentosa 80% ethanolic leaves used, the phenolic content was 10.82 ± 0.673 mg of Gallic Acid Equivalent (GAE)/g, the flavanoid content was 2.40 ± 0.501 mg of Quercetin Equivalent (QE) /g, the antioxidant capacity(FRAP) was 24.10± 0.747 mg of GAE/g of extract and 189.18 ± 0.26 ± 0.426 % inhibition for DPPH scavenging activity. Above result revealed that 80% ethanol extraction displayed Rhodomyrtus tomentosa leave extract contain high antioxidant capacity due to its phytochemical properties.

Key Words: Rhodomyrtus tomentosa, Antioxidant, 80% ethanol, UiTM, Phytochemical properties

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ACKNOWLEDGEMENTS

All Praise to The Almighty for His Shower of blessing, merciful and inspiration for me in completing this Final Year Project successfully

Foremost, I would like to express my profound attitude to my supervisor, Madam Evana Kamarudin who guide me during the study. Her sincere helps are really appreciated. Working closely with her give me the chance to appreciate her sharp intellect, warm and helpful nature.

During this difficult time, I was lucky to have my family and friends with me to give support and encouragements have been tremendous. Special thanks for my parents, Samsudin Bin Sajari and Lamah Binti Ahmad @Yahya, as well as goes to my friends. Their internal support has given me strength to fulfill my study.

This study cannot be success without valuable assist from staffs from Medical Laboratory Technology Department. Appreciation is gratefully extended to laboratory staff

My wholehearted thanks also go to all my group members for their support and providing me a conductive environment for this study.

Finally, I would like to thanks to all lecture, friends, and anyone who helped me directly or indirectly to make this thesis success.

Thank you

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TABLE OF CONTENTS

Pages

TITLE PAGE i

DICLARATION ii

INTELLECTUAL PROPERTIES iii

APPROVAL vi

ABSTRACT vii

ACKNOWLEDGEMENTS viii

TABLE OF CONTENTS ix

LIST OF TABLES xii

LIST OF FIGURES xiv

LIST OF ABBREVATION xv

CHAPTER

1. INTRODUCTION

1.1. Study Background 1

1.2. Problem Statement 2

1.3. Significant Of Study 2

1.4. Objective Of Study 3

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