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DENITRIFICATION PATH AND NUR BIOFILM PHASE OF MUNICIPAL SEWER NETWORKS USING ARTIFICIAL

WASTEWATER

By

SALWANIBT JAFFAR

Report is submitted as the requirement for degree of Bachelor Engineering (Hons) (Civil)

UNIVERSITITEKNOLOGI MARA MARCH 2004

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DECLARATION BY THE CANDIDATE

I'm Salwani bt Jaffar, UiTM no. 2001305123, confirm that the work is my own and that appropriate credit has been given where reference has been made to the work of others.

Yours truly,

(SALWAisTl BT JAFFAR) 15 APRIL 2004

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ABSTRACT

Extensive study on denitrification rates in the biofilm phase under sewer condition had been done. This paper will propose a study on denitrification path in the biofilm phases of municipal wastewater using of artificial wastewater under anoxic condition of excess electron acceptor and excess electron donor. In this study, experiment will conducted to establish the Nitrate Utilizing Rate (NUR) in biofilm phase of municipal wastewater using of artificial wastewater that represent by distilled water. The result is solely due only to biofilm as this study condition is using of artificial wastewater (distilled water) that not contain any organic compounds instead of the bulkwater that contain various of organic compounds which can utilized the nitrate rapidly until it depleted.. So, this study is a project based only on laboratory work. Result from this study can be used as a comparison to study done by Abdul-Talib (2002) and Johari and Abdul-Talib (2004) which gets the NUR from the differentiation of NUR in bulkwater and biofilm and NUR in bulkwater only.

Gas sample result from experiments conducted on 2 from 7 artificial wastewater samples have shown that the denitrification path in biofilm can be simplified by the reduction of nitrate to nitrogen with significant accumulation of nitrite. This is because the results have shown the insignificant of nitric oxide and nitrous oxide accumulations'. While, all of 7 liquid samples result, have shown two distinct stages during the denitrification processes.

The Nitrate Utilizing Rate (NUR) in the biofilm phase establish in this study ranges from 0.035 - 0.173 gN03T^/(m2h) with an average rate of 0.121 gN03"N/(m2h).

Keywords: Biofilm, artificial wastewater, denitrification path, Nitrate Utilizing Rate (NUR)

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ACKNOWLEDGEMENT

All praise to Allah SWT, lord of universe. The Merciful and the Gracious. Without His permissible, I cannot finish this report within time given. First of all, I want to express my gratitude to my supervisor, Prof. Madya Ir. Hj. Suhaimi B. Abdul Talib that had given me a change to do this topic of research. Without his guidance I cannot complete this study successfully.

Special thanks also to both of En. Suhaimi's research assistant, Noora Samsinar bt Johari and Siti Maizurah bt Misuan. Without their assist in preparing the instruments and materials needed for this project, I ensure that this study cannot be done and complete properly. I really "appreciate of all their help and teach from early stage until the final stage of this study.

Thank you also for all my friends for their helps and encouragements during preparing of this study. Not forget to my parents and siblings that give me spirit to complete this report. Last but not least, I hope this report can contribute some knowledge in order to go further study for this project.

SALWANIJAFFAR MARCH 2004

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TABLE OF CONTENTS

Description Pages

DECLARATION BY THE CANDIDATE I

ACKNOWLEDGMENT II TABLE OF CONTENTS III LIST OF FIGURES V LIST OF TABLES VI LIST OF ABBREVIATIONS VII

LIST OF APPENDICES VIII

ABSTRACT X

CHAPTER 1: INTRODUCTION

1.1 SEWER NETWORKS 1 1.2 PROBLEM STATEMENT 2 1.3 OBJECTIVE * 3

1.4 SCOPE OF WORK 4

CHAPTER 2: LITERATURE REVIEW

2.1 SEWERAGE SYSTEMS IN MALAYSIA 5

2.2 SEWER NETWORKS 6 2.3 PROCESSES IN SEWER NETWOKS 7

2.4 WASTEWATER TRANSFORMATION

2.4.1 Aerobic Transformation 10 2.4.2 Anaerobic Transformation 11 2.4.3 Anoxic Transformation 11

2.5 DENITRIFICATION 12 2.5.1 Denitrification Pathways in the BulkwateY

Phase of Wastewater 13

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