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IDENTIFICATION OF Salmonella enterica serovar Typhi PUTATIVE VIRULENCE FACTORS USING

A YEAST MORPHOLOGY ASSAY

PONG SZE YEN

UNIVERSITI SAINS MALAYSIA

2018

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IDENTIFICATION OF Salmonella enterica serovar Typhi PUTATIVE VIRULENCE FACTORS USING

A YEAST MORPHOLOGY ASSAY

by

PONG SZE YEN

Thesis submitted in fulfilment of the requirements for the degree of

Master of Science

September 2018

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ACKNOWLEDGEMENT

I would like to express my gratitude to everyone throughout my master study that had led to the accomplishment of this research project and thesis.

Lecturers,

Supervisor, Dr. Eugene Ong Boon Beng — for his valuable advices and guidance.

Co-supervisor, Prof Dr. Mohd Nazalan Bin Mohd Najimudin— for the sharing of ideas.

INFORMM lecturers — for their ideas and advice given during project presentations.

Typhoid lab members — for their assistance, technical help and support.

INFORMM students and staffs — for their friendship and help.

Universiti Sains Malaysia and INFORMM — for use of facilities and candidature matters.

FRGS grant — for the funding to support this research project.

MyBrains — for the scholarship that had supported my study for two semesters.

Lastly, I would like to express my sincere gratitude to my beloved family and friends for their support, encouragement and love throughout the days of conducting this project.

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iii

TABLE OF CONTENT

ACKNOWLEDGEMENT ii

TABLE OF CONTENT iii

LIST OF TABLES vi

LIST OF FIGURES vii

LIST OF SYMBOLS AND ABBREVIATIONS ix

ABSTRAK x

ABSTRACT xii

CHAPTER 1 - INTRODUCTION 1

1.1 General Introduction 1

1.2 Hypothesis and Aim of Thesis 2

CHAPTER 2 - LITERATURE REVIEW 4

2.1 Salmonella enterica serovar Typhi 4

2.2 Salmonella Infection 7

2.3 Host Specificity of S. Typhi 10

2.4 Virulence Factors 11

2.4.1 Salmonella Pathogenicity Island 13

2.4.2 Type III Secretion System 14

2.4.3 Other Virulence Factors 17

2.5 Universal Protein Resource 18

2.6 Yeast as Model System 18

2.6.1 Yeast Growth Inhibition as a Reporter for S. Typhi Virulence Factors

20 2.6.2 Yeast to Study S. Typhi Effector Proteins Subcellular

Localization

20 2.6.3 Yeast to Study Expressed S. Typhi Proteins mediated

Morphology Changes

22

CHAPTER 3 - CURATION OF PUTATIVE VIRULENCE FACTORS THROUGH BIOINFORMATICS ANALYSIS AND LITERATURE REVIEW

24

3.1 Introduction 24

3.2 Experiment Approach 25

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iv

3.3 Methods 25

3.1.1 Selection of VFs through Bioinformatics Analysis and Literature Review

25

3.4 Results and Discussion 26

CHAPTER 4 - HIGH-THROUGHPUT CLONING OF PUTATIVE VIRULENCE FACTOR GENES INTO YEAST EXPRESSION VECTOR

34

4.1 Introduction 34

4.2 Experiment Approach 35

4.3 Materials and Methods 36

4.3.1 General Methods, Strains and Plasmid 36

4.3.2 Oligonucleotides and Primers 37

4.3.3 Modification of pYES3/CT Plasmid 38

4.3.3(a) Adding of Multiple Cloning Sites and Homologous Region into pYES3/CT

38 4.3.3(b) Insertion of Green Fluorescent Protein into pYES3m

Plasmid

41 4.3.4 High-throughput Amplification of Putative VF genes 42

4.3.4(a) Primer Design and Preparation of Primers Mix for PCR Reaction

42

4.3.4(b) Polymerase Chain Reaction 44

4.3.5 High-throughput Homologous Cloning of Amplified Putative VF Genes into pYES3GFP

46 4.3.6 Validation of Clones through High-throughput Colony PCR 48 4.3.7 Extraction of Plasmids encoding Putative VF genes 49 4.3.8 High-throughput Transformation of Plasmids encoding Putative

VF Genes into Yeast

50

4.4 Results and Discussion 52

4.4.1 Modification of pYES3/CT Plasmid 52

4.4.2 High-throughput Amplification of Putative VF genes 53 4.4.3 Validation of Clones with Amplified Putative VF genes through

High-throughput Colony PCR

57 4.4.4 Extraction and High-throughput Transformation of Plasmids

encoding Putative VF Genes into Yeast

64

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CHAPTER 5 - DETERMINATION OF GROWTH INHIBITORY ACTIVITY OF THE PUTATIVE VIRULENCE FACTORS

67

5.1 Introduction 67

5.2 Experiment Approach 67

5.3 Materials and Methods 68

5.3.1 Growth of Yeast expressing GFP 68

5.3.2 Yeast Growth Inhibition Assay in 96-well Plate Format 68

5.3.2(a) Primary Screening 68

5.3.2(b) Secondary Screening 70

5.4 Results and Discussion 70

5.4.1 Growth of Yeast expressing GFP 70

5.4.2 Yeast Growth Inhibition Assay 71

5.4.2(a) Primary Screening 71

5.4.2(b) Secondary Screening 73

CHAPTER 6 – OBSERVATION OF MORPHOLOGICAL CHANGES IN YEAST CELLS THROUGH MICROSCOPY 78

6.1 Introduction 78

6.2 Experiment Approach 79

6.3 Methodology 79

6.3.1 Image Acquisition 79

6.3.1(a) Putative VF Proteins Subcellular Localization 80

6.3.1(b) Yeast Morphological Changes 80

6.4 Results and Discussion 84

6.4.1 Image Acquisition 84

6.4.1(a) Putative VF Proteins Subcellular Localization 90

6.4.1(b) Yeast Morphological Changes 97

CHAPTER 7 – CONCLUSION 101

REFERENCES 104

APPENDICES

LIST OF PUBLICATIONS AND PRESENTATIONS

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vi

LIST OF TABLES

Page Table 2.1 SPIs and their functions in virulence. 14 Table 3.1 Subcellular location of the selected 192 putative VFs

candidates.

29 Table 3.2 Summary of types and main functions of 192 selected

putative VFs candidates.

32

Table 4.1 Microbial strains and plasmids used. 36

Table 4.2 Oligonucleotides and primers used. 37

Table 4.3 Plasmid modification steps, components and conditions. 40 Table 4.4 Layout of putative VF genes in 96-well plate format. 43

Table 4.5 High-throughput PCR. 45

Table 4.6 High-throughput homologous cloning. 47

Table 4.7 High-throughput colony PCR. 49

Table 4.8 Yeast transformation components and conditions. 51 Table 4.9 Numbers of successful amplicons in each round of PCR

amplification.

53 Table 4.10 Amount of insert and plasmid used in each round of

transformation.

58

Table 5.1 Yeast growth assay. 69

Table 5.2 Putative VFs identified from yeast growth assay. 76 Table 6.1 Methods to measure yeast cells at various stages and shapes. 83 Table 6.2 Summary of protein subcellular localization in yeast. 92 Table 6.3 Summary of information of protein localized at cell

membrane, ER and cytoplasm.

95 Table 6.4 Summary of protein which caused filamentous growth in

yeast.

100 Table 6.5 The number of proteins according to yeast size. 100

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LIST OF FIGURES

Page

Figure 1.1 Outline and flow of the thesis. 3

Figure 2.1 Geographical distribution of typhoid incidence. 6

Figure 2.2 Overview of Salmonella infection. 9

Figure 2.3 Overview of S. Typhi virulence factors. 12 Figure 2.4 Structural overview of T3SS and location of the proteins. 16 Figure 2.5 Overview of Saccharomyces cerevisiae subcellular

compartments.

19 Figure 2.6 Bacterial effector protein localization in yeast. 21

Figure 2.7 Normal and filamentous yeast. 23

Figure 3.1 Summary of methodology and results for the selection of putative VFs candidates through bioinformatics analysis and literature review.

28

Figure 4.1 pYES3/CT plasmid modification. 39

Figure 4.2 Insertion of GFP gene into modified pYES3/CT plasmid. 41 Figure 4.3 Example of primers design for PCR amplification and the

expected PCR product.

42 Figure 4.4 Schematic diagram of plasmid modification. 52 Figure 4.5 DNA gel electrophoresis of plate 1 PCR amplicons in the

first round PCR amplification.

54 Figure 4.6 DNA gel electrophoresis of plate 2 PCR amplicons in the

first round PCR amplification.

55 Figure 4.7 DNA gel electrophoresis of second and third round PCR

amplification.

56 Figure 4.8 DNA gel electrophoresis of colony PCR screening for

colonies cloned with selected genes from plate 1 in first round cloning.

59

Figure 4.9 DNA gel electrophoresis of CPCR screening for colonies cloned with selected genes from plate 2 in first round cloning.

60

Figure 4.10 DNA gel electrophoresis of colony PCR screening of plate 1 for second round cloning.

61

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Figure 4.11 DNA gel electrophoresis of colony PCR screening of plate 2 for second round cloning.

62 Figure 4.12 DNA gel electrophoresis of colony PCR screening for third

round cloning.

63 Figure 4.13 DNA gel electrophoresis of plasmid encoding selected

genes from plate 1.

65 Figure 4.14 DNA gel electrophoresis of plasmid encoding selected

genes from plate 2.

66

Figure 5.1 Growth curve of pYES3m and pYES3GFP. 70

Figure 5.2 Result of 190 putative VFs growth percentage (%) at 36 h. 72 Figure 5.3 Result of 18 putative VFs growth percentage (%) at 36 h. 75 Figure 6.1 Steps to measure yeast cell size using ImageJ. 82 Figure 6.2 Plate 1 bright-field channel images merged with

fluorescent channel images.

86

Figure 6.3 Plate 1 fluorescent channel images. 87

Figure 6.4 Plate 2 bright-field channel images merged with fluorescent channel images.

88

Figure 6.5 Plate 2 fluorescent channel images. 89

Figure 6.6 Subcellular localization of GFP-tagged proteins. 93

Figure 6.7 Filamentous yeast cells. 99

Figure 7.1 Overall summary of findings in this study. 102

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LIST OF SYMBOLS AND ABBREVIATIONS

% Percentage

μl Microliter

μm Micrometer

6×His-tag Polyhistidine-tag

bp Base pair

DMSO Dimethyl sulfoxide

DNA Deoxyribonucleic acid

E. coli Escherichia coli

ER Endoplasmic reticulum

h Hour

HR Homologous region

HT High-throughput

g Gram

GFP Green fluorescent protein

kbp Kilo-base pair

LB Luria broth

LPS Lipopolysaccharides

M Molar

MB Megabyte

MCS Multiple cloning site

min Minutes

ml Milliliter

mM Millimolar

OD Optical density

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ORF Open reading frame

PCR Polymerase chain reaction

s Seconds

SC Synthetic complete medium

S. cerevisiae Saccharomyces cerevisiae

SCV Salmonella-containing vacuole

Sif Salmonella-induced filaments

SPI Salmonella pathogenicity Island

S. Typhi Salmonella enterica serovar Typhi

T3SS Type III secretion system

VF Virulence factor

YPD Yeast extract-Peptone-Dextrose medium

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PENGENALPASTIAN FAKTOR VIRULENS PUTATIF Salmonella enterica serovar Typhi MENGGUNAKAN ASAI MORFOLOGI YIS

ABSTRAK

Demam kepialu adalah jangkitan bakteria bersimtomatik yang disebabkan Salmonella enterica serovar Typhi (S. Typhi) yang biasanya berlaku akibat system sanitasi yang tidak baik. Pengenalpastian faktor virulens (VF) novel S. Typhi akan membolehkan pemahaman yang lebih baik mengenai patogenesis jangkitan demam kepialu dan membantu pengurusan penyakit ini. Terdapat VF yang masih tidak dikenalpasti dan untuk VF yang telah diketahui, peranan VF tersebut dalam patogenesis masih belum difahami sepenuhnya. Dalam kajian ini, yis Saccharomyces cerevisiae (S. cerevisiae) digunakan sebagai organisma model untuk mengenal pasti VF S. Typhi putatif. Sebanyak 192 VF putatif dipilih daripada proteom S. Typhi CT18 melalui semakan kajian literatur dan carian pangkalan data atas talian. Kemudian, gen VF putatif terpilih diklon dan diekspres dalam yis menggunakan format plat 96-telaga.

Sebanyak 190 VF putatif diklonkan ke dalam plasmid yang mempunyai protein fluoresen hijau dan diekspres dalam yis. Dengan menggunakan asai pertumbuhan mikroplat yang mengukur kepadatan optik kultur cair, sembilan VF putatif didapati merencat pertumbuhan yis. Lokasi protein VF putatif dalam yis diperhatikan dan dikategorikan, satu protein efektor berlokasi di membran sel dan empat protein efektor masing-masing berlokasi di retikulum endoplasma dan sitoplasma. Asai morfologi yis menunjukkan bahawa lima VF putatif menjalani pertumbuhan filamen dan 98 VF putatif menunjukkan perubahan saiz sel yang ketara. Kesimpulannya, pemerhatian morfologi yis adalah penting untuk mengenal pasti VF putative yang boleh

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menyebabkan gangguan pada proses intrasel tetapi tidak semestinya menghalang pertumbuhan yis.

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IDENTIFICATION OF Salmonella enterica serovar Typhi PUTATIVE VIRULENCE FACTORS USING A YEAST MORPHOLOGY ASSAY

ABSTRACT

Typhoid fever is a symptomatic bacterial infection caused by Salmonella enterica serovar Typhi that usually occurs due to poor sanitation and hygiene. The identification of novel virulence factors (VF) of S. Typhi will allow for better understanding of the pathogenesis of typhoid infection and potentially help disease management. There are still unidentified VFs and for those already reported, their roles in pathogenesis are still not completely understood. In this study, Saccharomyces cerevisiae was used as a model organism to identify putative S. Typhi. A total of 192 putative VFs were selected from the proteome of S. Typhi CT18 through cross- reference with published literature and database searches. Then, the selected VF genes were cloned and expressed in yeast in a 96-well plate format. A total of 190 putative VFs were cloned into plasmid with green fluorescent protein and expressed in yeast.

By using a microplate growth assay that measures optical density of liquid culture, nine putative VFs were found to inhibit the yeast growth. Then the putative VF proteins’ localization in yeast were observed and categorised, one effector protein localized at cell membrane and four effector proteins each at endoplasmic reticulum and cytoplasm. Result of the morphology assay showed that five of the putative VFs undergo filamentous growth and 98 putative VFs showed significant cell size changes.

In conclusion, yeast morphology observation is essential to identify putative VFs that can interfere with yeast intracellular process but not necessarily inhibit yeast growth.

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1 CHAPTER 1 INTRODUCTION 1.1 General Introduction

Salmonella enterica serovars Typhi (S. Typhi) is one of the oldest human diseases and believed to cause the “plague of Athens” during the Peloponnesian war of 430 B.C (Galan, 2016). S. Typhi is a human host-restricted organism that causes typhoid fever. It remains a major global health problem with the disease burden of 9.9 to 24.2 million cases and 75,000 - 208,000 deaths per year (Das et al., 2017). High areas of typhoid endemicity include South-central Asia, South-east Asia, and Southern Africa (Mogasale et al., 2014).

Virulence factors (VF) are molecules produced by pathogens that act individually or together to facilitate the replication and dissemination of the bacterium within a host. These factors are either secretory, membrane associated or cytosolic in nature (Sharma et al., 2017). Research efforts have been focused to understand the pathogenicity of S. Typhi and the identification of S. Typhi VFs can clarify S. Typhi pathogenesis mechanisms and thus provide a roadmap for future treatment strategies and prevention of typhoid fever (Hurley et al., 2014).

In this study, the yeast Saccharomyces cerevisiae (S. cerevisiae) was used as a model organism to study S. Typhi VFs. S. cerevisiae has been shown to be a good model for characterizing bacterial VFs although it cannot be used as a physiological model for human infections (Aleman et al., 2009). The use of yeast cells as a model of eukaryotic cells can reduce time and cost needed for this study (Aleman et al., 2009;

Slagowski et al., 2008). When VFs are expressed in yeast cells, it can interfere with normal cellular processes of yeast cells and lead to growth inhibition. Thus, inhibition

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in yeast growth can serve as an indicator to screen for the presence of VFs (Slagowski et al., 2008). Additionally, previous studies also showed that VFs when expressed in yeast can cause morphological changes to their cells (Aleman et al., 2009; Lesser &

Miller, 2001).

The putative VFs genes were selected through bioinformatic analysis and literature review. Then the selected genes were cloned into plasmid that will allow for green fluorescent protein (GFP) tagging. Protein expression was carried out in a high- throughput format. This was followed by screening of putative VFs using a yeast growth inhibition assay and yeast morphology observation. The subcellular localization of the selected VF proteins were also determined. The overall approach used is outlined in Figure 1.1.

1.2 Hypothesis and Aim of Thesis

Not all S. Typhi VFs have been identified, and for those identified not all their functions are known. Thus, the aim of this study is to identify putative S. Typhi VFs by identifying proteins that can cause morphological change in S. cerevisiae cells.

Specific aims are:

1. To curate a list of putative S. Typhi VFs through bioinformatics analysis and literature review.

2. To develop a high-throughput cloning and protein expression platform.

3. To determine the growth inhibitory activity of the putative VFs.

4. To identify morphological changes caused by VFs in yeast cells through microscopy.

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3 Chapter 3

Curation of putative VFs through bioinformatics analysis and literature review

- Analysis of S. Typhi proteome using online database - Cross-reference of putative VFs from literature and

databases

Chapter 4

High throughput cloning of putative VF genes into yeast expression vector

- Cloning using homologous recombination method - Transformation of the cloned vector into yeast

Chapter 5

Determination of the growth inhibitory activity of the putative VFs

- Expression of proteins in yeast and examining growth inhibition through liquid growth assay

Chapter 6

Observation of morphological changes in yeast cells through microscopy

- Yeast morphology observation - Protein subcellular localization

Figure 1.1 Outline and flow of thesis.

Experimental approach taken to identify S. Typhi VFs and study of its effect on cellular morphology.

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4 CHAPTER 2 LITERATURE REVIEW 2.1 Salmonella enterica serovar Typhi

Salmonella enterica serovar Typhi (S. Typhi) is a Gram-negative, facultative anaerobic and flagellated bacilli from the family of Enterobacteriaceae (Lamas et al., 2018). The genus of Salmonella contains 2600 serotypes (termed “serovar”) and classified into 2 species, which is Salmonella enterica and Salmonella bongori (Ryan et al., 2017).

Based on the Kauffman-White classification scheme, S. enterica is further divided into six distinct subspecies which are enterica, salamae, arizonae, diarizonae, houtenae and indica. Of these six subspecies, only subspecies enterica is associated with disease in warm-blooded animals (Baddam et al., 2014).

Human-adapted S. Typhi is a worldwide foodborne pathogen and the causative agent of the typhoid fever (Lamas et al., 2018). Typhoid fever remains a common infection in regions with poor economic development and limited public health infrastructure (Dougan & Baker, 2014). It is transmitted through unhygienic water sources, poor hygiene practices and unsanitary living conditions (Mogasale et al., 2014; Wain et al., 2015).

Following infection through ingestion, symptoms begin after an incubation period that usually lasts 7-14 days. The onset of diseases is marked by fever as high as 39° to 40°C. Followed by abdominal distension, constipation, headache, rash, malaise, loss of appetite, nausea, vomiting, hepatosplenomegaly and leukopenia (Akinyemi et al., 2005). Some serious complications such as gastrointestinal bleeding,

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intestinal perforation and typhoid encephalopathy may occur in 10-15% of typhoid patients (Non et al., 2015).

It is estimated that 26.9 million cases of disease per year reported worldwide, resulting in 216 000 deaths (Buckle et al., 2012). High incidence areas of typhoid endemicity include South-central Asia, Southeast Asia, and Southern Africa (Figure 2.1) (Mogasale et al., 2014). Although the global burden of typhoid fever has reduced, the emergence of multi-drugresistant S. Typhi (MDRST) is still a serious health problem worldwide (Chiu et al., 2002).

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Figure 2.1 Geographical distribution of typhoid incidence.

Typhoid have high incidence at South-central Asia, Southeast Asia, and Southern Africa (adapted from Mogasale et al., 2014).

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7 2.2 Salmonella Infection

S. Typhi enter human digestive system through contaminated food or water.

After surviving the low pH of stomach acid,S. Typhi come in contact with the epithelial cells of intestines. Salmonella preferentially invades the intestinal barrier through microfold (M) cells and later disseminated into lymph nodes, spleen and liver (Haraga et al., 2008; Suarez & Russmann, 1998) (Figure 2.2A).

Internalization of Salmonella into host cells occur depending on the invading cells type, such as phagocytic and non-phagocytic cells using a type III secretion system (T3SS1) (Figure 2.2B). Phagocytosis in S. Typhi involves multiple receptors to activate different signalling pathways in the phagocyte (Ibarra & Steele-Mortimer, 2009). Whereas, T3SS1 mediated invasion is a highly specific process that is regulated by the expression of a number of effector proteins (SipA, SipC, SopB/SigD, SopD, SopE2 and SptP) (Kage et al., 2008).

When contact with the intestinal epithelial cells, S. Typhi inject T3SS1, which is a needle like complex into the host cells membranes and assemble a channel between the S. Typhi and cell membranes. Translocated proteins will enter the host cell cytosol through the T3SS1. These protein will trigger a complex set of signalling events in the host cell which cause cytoskeletal rearrangements (Schmidt & Hensel, 2004). The rearrangement of the host cells actin cytoskeleton will result in membrane ruffling that uptake the S. Typhi into the host epithelial cells (Boumart et al., 2014).

Besides phagocytosis and T3SS1 mediated invasion, fimbriae and adhesins on the surface of S. Typhi also facilitate the attachment and internalization through T3SS- 1 independent process (Ibarra & Steele-Mortimer, 2009).

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Once the epithelial barrier has been breached, S. Typhi will activate various virulence mechanisms for survival in the host cells environment. This promotes bacterial replication and subsequently invade into the reticuloendothelial system (RES) (Haraga et al., 2008).

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9 Figure 2.2 Overview of Salmonella infection.

A, Ingested Salmonella invade host intestinal epithelial cells through M cell and cross the intestinal barrier before disseminated to other parts of body (adapted from(Haraga et al., 2008). B, Different methods of internalization of Salmonella into host cells (adapted and modified from(Ibarra & Steele-Mortimer, 2009).

A

B

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10 2.3 Host Specificity of S. Typhi

S. Typhi is human restricted pathogens which only develop and caused diseases in human host. Studies showed that chimpanzees can be infected with S.

Typhi but do not develop typhoid fever. Indeed, the S. Typhi-infected animals developed symptoms which are more “nontyphoidal Salmonella” infection alike than those of typhoid fever (Song et al., 2013). It is hypothesized that typhoid toxin which only encoded by typhoidal Salmonella serovars S. Typhi and S. Paratyphi play important role in the development of typhoid fever (Galan, 2016).

Glycoarray analysis showed typhoid toxin prefer to bind to termini with the consensus sequence Neu5Ac2-3Galβ1-3 and does not bind to termini with N- glycolylneuraminic acid (Neu5Gc) (Galan, 2016). This results provided perception for S. Typhi human-host specificity because sialoglycans on human cells were terminated in N-acetylneuraminic acid (Neu5Ac) (L. Deng et al., 2014). Whereas for other primates and mammals, it is terminated in Neu5Gc. This difference was caused by a mutation in human gene which emerged after hominids separated from other primates.

This mutation encodes enzyme CMP-N-acetylneuraminic acid hydroxylase (CMAH), which converts Neu5Ac to Neu5Gc. This insight is strengthed when typhoid toxin is capable to induce typhoid fever symptoms in mice. The expression pattern of CMAH in mice is variables, displaying sialoglycans terminated in both Neu5Ac and Neu5Gc (Hedlund et al., 2007). Thus, these explaining reasons S. Typhi only cause typhoid fever in human host.

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11 2.4 Virulence Factors

Virulence factors (VF) are molecules produced by pathogens to promote the invasion and survival of pathogens in the host cells environment. The ability of bacteria to infect a host determined by multiple virulence factors acting individually or together at different stages of infection (Wu et al., 2008). Some VFs regulate each other or antagonistically to promote infections. VFs secreted during late stage of infection may modulate the functions of those secreted during early stage, changing the bacterial virulence strategy to promote infection. Also, a single VF can have multiple functions and host targets, such as S. Typhi SopB which involve in rearrangement of actin cytoskeleton and also stimulating fluid secretion which cause diarrhoea (Hurley et al., 2014; Ong et al., 2010; Sharma et al., 2017).

Identification of S. Typhi VFs are important to understand the mechanism of S. Typhi pathogenesis and their interactions with the host, thus provide a roadmap for future treatment strategies and prevention of typhoid fever. Here are some example of Salmonella VFs: Pathogenicity islands, fimbriae, flagellar, Vi antigen, lipopolysaccharides and many more (Figure 2.3) (Wu et al., 2008).

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Figure 2.3 Overview of S. Typhi virulence factors.

Different types S. Typhi virulence factors (adapted and modified from(de Jong et al., 2012).

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13 2.4.1 Salmonella Pathogenicity Island

Majority of the Salmonella VFs are clustered on Salmonella Pathogenicity Islands (SPI) on the chromosome. To date, there are 21 SPI reported. However, not all SPI are present in all Salmonella serotypes, only SPI-1, SPI-2, SPI-3, SPI-4 and SPI- 5 were present in all serotypes. Based on the complete genome sequence of S. Typhi CT18, there are at least 5 more regions can be designated as SPIs (Parkhill et al., 2001).

Salmonella pathogenicity island 1 (SPI-1) is 40 kb DNA region, bearing the genetic information encoding for type III secretion system (T3SS1) at centisome 63 at Samonella chromosome (Galan, 2001). T3SS1 play important roles during interaction between the Salmonella and host intestinal epithelial cells helping the entry into host cells and initiation of the diseases. Whereas, SPI-2 located at the centisome 30.7 of the bacterial chromosome and encoding for two-component regulatory system and T3SS2 (Schmidt & Hensel, 2004)

Other than these two major SPIs, other SPIs (SPI-3, SPI-4, SPI-5, SPI-7, SPI-8, SPI-9 and SPI-10) have also been identified to play important roles in virulence and survival of the bacteria (Table 2.1)(Ong et al., 2010). However, most of the SPIs function are not well studied yet.

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Table 2.1 SPIs and their functions in virulence (adapted from (Edwards et al., 2000; Ong et al., 2010; Valdez et al., 2009).

SPI Encode Function in virulence

SPI-1 - T3SS1 Invasion of epithelial cells

SPI-2 -Two-component regulatory system

-T3SS2

Survival of S. Typhi inside Salmonella Containing Vacuole (SCV)

SPI-3 - Mg2+ uptake system Salmonella survival in the SCV SPI-4 - Nonfimbrial adhesin Bacterial adherence to the apical

surface of polarized cells SPI-5 - Effector proteins that are

secreted by SPI-1 and SPI-2 T3SS

Salmonella enteropathogenesis

SPI-6 - saf and tcf fimbrial operons Bacterial adherence SPI-7 - Vi biosynthetic genes

- Type IV fimbrial operon

Host cell cytoskeletal rearrangement SPI-8 - Colicin/pyocin Kill other bacteria to compete for

nutrients SPI-9 - Type I secretion system

- Repeats-in-toxin (RTX) proteins

Adhesion in intestine

SPI-10 - sef fimbrial operon Colonization and penetration of the intestinal barrier.

2.4.2 Type III Secretion System

T3SSs are supramolecular complexes that play a major role in the invasion of host cells, it serves as a channel for the effector proteins to enter into host cytosol (Ibarra & Steele-Mortimer, 2009). The T3SS proteins can be categorized into export apparatus, needle complex, translocons, regulators, effectors, and chaperones (Figure 2.4).

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SPI-1 encoded T3SS (T3SS1) genes are expressed when S. Typhi contact with host epithelial cells under regulation of T3SS1 regulatory proteins (InvF, HilA, HilC and HilD) (Srikanth et al., 2011). The translocated effector proteins will enter into to T3SS1 needle complex (PrgI and InvG) through the export apparatus (SpaO, PrgH and PrgK) which assembled at the S. Typhi inner membrane (burkinsaw). SpaS and InvA function as gate for controlling the access into the needle complex. On the other hand, translocon proteins (SipB, SipC and SipD) which located at the other end of needle complex and responsible for forming pores on host cell membranes (Haraga et al., 2008). Once the effector proteins enter into host cytosol, it will induce host actin cytoskeleton rearrangement to form a ‘pocket’ to engulf and uptake the S. Typhi into the host cells (Ramos-Morales, 2012).

The SPI-2 T3SS genes are only expressed inside host SCV and regulated by three important two-component regulatory systems including SpiR/SsrB, PhoP/PhoQ and EnvZ/OmpR in response to acidic pH and nutrient limitation in the SCV(Ramos-Morales, 2012). The activated SPI-2 T3SS facilitated the translocation of the effector proteins across SCV membrane into host cells through the needle complex (SsaG) (Kaur & Jain, 2012). After entering into host cells, these VFs will interfere host cellular processes for replication and survival in the host cells (Kaur & Jain, 2012).

For instance, SpiC, a protein translocated into host macrophages cytosol will interfere normal secretory pathway of the host cell which protects the Salmonella for bactericidal compounds (Haraga et al., 2008).

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Figure 2.4 Structural overview of T3SS and location of the proteins.

The export apparatus located within the inner membrane of Salmonella. Needle components are assemble into the needle complex passing through Salmonella outer membrane into extracellular environment. The transcolon components form pores on host cell membrane and transferring Salmonella effector proteins into the host cell cytoplasm. SPI-1 encoded T3SS (T3SS1) translocate VFs from bacterial cytoplasm to host cells, across the host plasma membrane whereas SPI-2 T3SS (T3SS2) is expressed inside SCV and translocate VFs to host cytoplasm, across the vacuolar membrane (adapted and modified from(Ramos-Morales, 2012).

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17 2.4.3 Others Virulence Factors

Besides SPI, other VFs of Salmonella also play important roles in bacterial pathogenesis. Some example of the VFs are fimbriae and flagellar, Vi antigen, lipopolysaccharides (Wu et al., 2008).

Flagella are complex motility structures which have been associated with virulence in many pathogens, including Salmonella. Reduces adherence ability to human intestinal epithelial cell lines are show in flagellar mutants strains, suggesting that flagella are important in adherence of Salmonella to intestinal epithelial cells during invasion (Dibb-Fuller et al., 1999).

Fimbriae are hair-like appendages that protrude outside the bacterial surface membrane which play important role in biofilm formation, adhesion and colonization of bacteria in the host cells such as epithelial, lymphoid and endothelial cells (Proft &

Baker, 2009).

Vi antigen found to maintain bacterial survival during host immune responses by preventing recognition of host pattern recognition receptors (PRRs) (de Jong et al., 2012). Studies have shown loss of Vi antigen reduced salmonella virulence in mice thus suggested the importance of Vi antigen in bacteria pathogenesis (Raffatellu et al., 2006).

Lipopolysaccharides (LPS) is a major component of the outer membrane of Salmonella. It consists a hydrophobic region (lipid A) that anchors it to the bacterial outer membrane, a non-repeated core oligosaccharide, and a repeated polysaccharide (O antigen) (Raetz & Whitfield, 2002). LPS helps to protect Salmonella from the acidic environment of the gastrointestinal tract and important in host-pathogen interactions with host immune system.

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18 2.5 Universal Protein Resource

Universal Protein Resource (UniProt) is a freely accessible database, combining the view of protein sequence and functional annotations through integration of data from different resources (UniProt Consortium, 2018). UniProt can be reached at https://www.uniprot.org. UniProt is composed of several important component parts and each designed for different uses, the UniProt Knowledgebase (UniProtKB), the UniProt Reference Clusters and the UniProt Metagenomic and Environmental Sequence Database (UniProt, 2013).

2.6 Yeast as Model System

Saccharomyces cerevisiae or usually known as baker’s yeast has been widely used as a model organisms for more than 50 years (Duina et al., 2014) (Figure 2.5).

Up to 30% of genes involve in human disease have orthologs in the yeast sequences and thus conserving most eukaryotic cellular process (Franssens et al., 2013; Karathia et al., 2011). With these criteria, the use of yeast has been shown to be a powerful approach for understanding the interactions of bacterium and its host, providing further pieces of knowledge of their enzymatic functions (Curak et al., 2009; Siggers & Lesser, 2008; Valdivia, 2004).

Previous studies showed yeast was used as a surrogate system to characterize the activities of the Zika viral genome (Li et al., 2017). The studies showed that the Zika viral protein subcellular localization was overall parallel to its predicted protein structure. Some of the Zika viral proteins showed various levels of cytopathic effects such as regulate cell growth, induced hypertrophy, or cellular oxidative stress which lead to cell death. So, yeast can be used as a model to study VFs either with yeast cell growth, cell morphology and also the protein subcellular localization.

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Figure 2.5 Overview of Saccharomyces cerevisiae subcellular compartments.

Yeast cells contain various subcellular (adapted from(Walker, 2016).

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2.6.1 Yeast growth Inhibition as a Reporter for S. Typhi Virulence Factors There are increasing studies revealed that heterologous expression of bacterial effector proteins in yeast will lead to yeast growth inhibition (Siggers & Lesser, 2008).

For example, effector proteins from Pseudomonas aeruginosa, Shigella flexneri, Salmonella Typhimurium, Legionella pneumophila and Yersinia species have been observed to inhibit growth when expressed in yeast (Siggers & Lesser, 2008). These growth inhibition is the indirect result of effector proteins interfere with the yeast cytoskeleton, organelle membranes, or specific signalling pathways (Popa et al., 2016).

Thus proving that yeast growth inhibition is a sensitive and specific reporter to screen for bacteria effector proteins or virulence factors which can perturb host cellular processes (Aleman et al., 2009).

2.6.2 Yeast to study S. Typhi Effector Proteins Subcellular Localization

Previous studies suggested that bacterial effector heterologous expressed in yeast could accurately reflect their localization when injected into host cells during invasion (Siggers & Lesser, 2008). This shows that the molecular mechanisms of effector targeting are likely conserved from yeast to human. For example, Salmonella effector proteins SopE2 are localized at yeast plasma membrane and PipB targeted to endoplasmic reticulum, precisely matching the localization which previously reported in mammalian cells (Weigele et al., 2017).

Few studies visualized the protein subcellular localization by fusing the protein of interested with fluorescent proteins. Green fluorescent protein (GFP) is one of the fluorescent proteins which is widely used to tagged proteins of interest. The subcellular localization of the effector proteins can be categorised by the GFP localization pattern or with the help of other organelles fluorescent stains as guides (Weigele et al., 2017)

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(Figure 2.6). This localization patterns of proteins in yeast providing useful tool to identify the subcellular localization and molecular mechanisms of the proteins (Lippincott-Schwartz et al., 2003).

Figure 2.6 Bacterial effector protein localization in yeast.

Subcellular localization of GFP tagged proteins in yeast cells. A, endoplasmic reticulum; B, cytoplasm; C, nucleus D, vacuoles E, cell membrane (adapted and modified from(Henke et al., 2011; Weigele et al., 2017).

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2.6.3 Yeast to Study the Expressed S. Typhi Proteins mediated Morphology Changes

Besides using subcellular localization to study eukaryotic cellular processes, another approach is to screen for effector proteins that can mediate the yeast morphology changes. For example, many pathogens interfere with the host actin cytoskeleton during the invasion process. Such as Shigella and Salmonella which mediate their uptake into normally nonphagocytic cells by delivering effector proteins that induce the formation of membrane ruffles in the cells (Cossart & Sansonetti, 2004;

Ly & Casanova, 2007). Both yeast and mammals conserved the molecular switches Rho GTPases that regulate the actin cytoskeleton (Etienne-Manneville & Hall, 2002).

Some effector proteins will cause different responses when introduced into different cell types, such as expression of Salmonella SopE2 that activates Cdc42, results in formation of membrane ruffles in mammalian cells (Rho GTPases pathway).

While in yeast, it is associated with activation of the filamentous growth pathway (MAPK pathway), causing the yeast to filament (Figure 2.7) (Rodriguez-Pachon et al., 2002).

Besides the filamentous shape of yeast cells, expression of bacterial proteins also caused changes in yeast cell size, such as enlargement or reduce in yeast cell sizes, due to the disruption of the yeast intracellular processes by bacterial effector proteins.

The changes in cell size will indirectly changing the cell volume or surface area which will interfere with cell normal metabolic flux, biosynthetic capacity, nutrient exchange and most importantly cell division process (Marshall et al., 2012).

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Figure 2.7 Normal and filamentous yeast

Scanning electron micrographs showing normal and filamentous yeast cells which expressing Salmonella SopE2 (adapted and modified from(Rodriguez-Pachon et al., 2002).

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24 CHAPTER 3

CURATION OF PUTATIVE VIRULENCE FACTORS THROUGH BIOINFORMATICS ANALYSIS AND LITERATURE REVIEW 3.1 Introduction

VFs are molecules produced by pathogens to assist the colonization and survival of pathogens in the host cells environment. These factors are either secretory, membrane associated or cytosolic (Sharma et al., 2017). All these factors are working together or individually during different stages of infection to ensure the establishment of the diseases (Wu et al., 2008). Secretory factors play important roles in helping the bacterium to survive the host immune response and also host cell–bacteria interactions.

Whereas the membrane associated VFs are involved in adhesion and invasion of host cells. The cytosolic factors facilitate the bacterium metabolic and physiological processes (Sharma et al., 2017).

UniProtKB/Swiss-Prot contain manually curated records with information extracted from literature and curator-evaluated computational analysis.

UniProtKB/Swiss-Prot currently contains about half a million sequences and continues to grow as new proteins are experimentally reported (UniProt, 2014). Whereas for the sequences which are unreviewed collected in UniProtKB/TrEMBL. The entries of UniProtKB/TrEMBL are automatically generated annotation supplemented by computationally analyzed records (Poux et al., 2017). Both (UniProtKB/Swiss Prot) and (UniProtKB/TrEMBL) provide protein information including biological process, molecular function and cellular component (UniProt, 2013). Therefore, UniProtKB is a useful online tool to facilitate the process of acquiring of protein sequences and functional information of S. Typhi proteomes in this study.

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Besides UniProt, VF databases such as Virulence Factor Database (VFDB) and PathoSystems Resource Integration Center (PATRIC) were also referred when curating a list of possible VFs for testing. VFDB (http://www.mgc.ac.cn/VFs/) is a free online database that serves to provide a source for scientists to access to current knowledge of VFs from various bacterial pathogens (Chen et al., 2013). On the other hand, the PATRIC database provide complete bioinformatics resource for pathogens, including genomics, proteome and metabolic pathway data to facilitate fundamental biomedical research on bacterial caused diseases (Wattam et al., 2017).

3.2 Experimental Approach

The UniProtKB database was used to search and download S. Typhi complete proteome. Possible VFs were selected by analysing the proteins based on protein general annotation, gene ontology (GO) provided in the database and further cross- referenced to putative VFs reported in literature and virulence databases (VFDB, PATRIC).

3.3 Methods

3.3.1 Selection of VFs through Bioinformatic Analysis and Literature Review S. Typhi proteins with general annotation including protein names, gene names, biological processes, functions, subcellular location and length were downloaded from UniProtKB (http://www.uniprot.org/uniprot/?query=

proteome:UP000000541+AND+proteomecomponent:%22Chromosome%22) in Microsoft Excel file format. A list of possible VFs were selected through the analysis of the proteins based on the general annotation and filtered with keywords related to VFs. The keywords used were pathogenesis, virulence, Pathogenicity Island, Type III secretion, secreted, flagella, fimbria, toxin, Vi polysaccharide, lipopolysaccharide (LPS), adhesion and invasion. The selected VFs were then cross-referenced with

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